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KMID : 0387420190300040357
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Korean Society of Oriental Neuropsychiatry
2019 Volume.30 No. 4 p.357 ~ p.370
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Effects of Hominis Placenta Pharmacopucture in a Chronic Physical Stress Rat Model on Changes in Behavior, Weight, Sleep-Related Hormones, Inflammatory Cytokines, and Anti-Oxidant Enzymes
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Ha Ji-Won
Lee Yu-Mi
Na Chang-Su
Sakong Jong-Won
Kim Bo-Kyung
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Abstract
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Objectives: This study was designed to examine the effect of Hominis Placenta Pharmacopuncture on the change in behavior, sleep-related hormones, inflammatory cytokines, anti-oxidants, weight, blood, and serum on rats given chronic physical stress.
Methods: Wistar rats older than age 10 weeks were used in this experiment. They were divided into six groups. The normal group was not given stress. The control group was given only chronic physical stress and no treatment. The positive control group was given chronic physical stress and treated with zolpidem. Three Hominis Placenta Pharmacopuncture (HPP) groups were given chronic physical stress, then treated with different concentrations of HPP; HPP-0.5× (0.5 times diluted), HPP-1× (undiluted) and HPP-2× (2 times condensed). The changes of values of Nestlet Shredding results, weight, Melatonin, Gamma-aminobutylic Acid (GABA), Tumor Necrosis Factor Alpha (TNF-α), Interleukin-6 (IL-6), Superoxide dismutase (SOD), glutathione peroxidase (GPx), AST, ALT, BUN, Creatinine, and leukocytes were observed during the experiment.
Results: The changes in chronic physical stress of the rat model were as follow. The Nestlet Shredding result increased in the control group compared to the normal group (p＜0.05), and decreased in the HPP-1× and HPP-2× compared to the control group (p＜0.05). The amount of weight gain showed increasing tendency in the HPP-2× compared to the control group since the second week. The GABA increased (p＜0.05) and Melatonin also showed certain increasing tendency in the HPP-1× and HPP-2× compared to the control group. The TNF-α and IL-6 increased in the control group compared to the normal group (p＜0.01), and decreased in all the HPPs compared to the control group (p＜0.05). The SOD level decreased in the control group compared to the normal group (p＜0.01), and increased in all the HPPs compared to the control group (p＜0.05). GPx, AST, ALT, Bun, Creatinine and leukocytes showed no noticeable difference among all groups.
Conclusions: Hominis Placenta Pharmacopuncture was effective in increasing weight, GABA, Melatonin, SOD, and decreasing Nestlet Shredding and inflammatory cytokines, suggesting that it consequently facilitates in relieving physical stress.
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KEYWORD
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Hominis Placenta, Pharmacopuncture treatment, Chronic stress, Antioxidants, Melatonin, Cytokines
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